Efficient Virus-Induced Gene Silencing in Ilex dabieshanensis Using Tobacco Rattle Virus

Ilex dabieshanensis is not only an important ornamental plant, but can also be used to produce Kuding tea, owing its lipid-lowering and anti-inflammatory medicinal properties. The genetic transformation of I. currently difficult, which restricts functional gene studies molecular breeding research on this species. Virus-induced silencing (VIGS) a powerful tool for determining functions in plants. present study reports the first application VIGS mediated by tobacco rattle virus (TRV) vector dabieshanensis. We tested efficiency system silence Mg-chelatase H subunit (ChlH) through agroinfiltration. agroinfiltrated leaves exhibited typical yellow-leaf phenotype ChlH at 21 days post infiltration. Endogenous expression levels plants were all significantly lower than that mock-infected control Overall, our results indicated TRV-based efficiently genes dabieshanensis, will contribute efficient genomics